ISO 17721-1-2021 pdf free download – Quantitative determination of antibacterial activity of ceramic tile surfaces — Test methods — Part 1: Ceramic tile surfaces with incorporated antibacterial agents.
Other culture media may be selected based on the requirements for the bacteria selected for the testing.Details of the selected media shall be included in the test report under culture conditions.
Prepared culture media may be stored at 5 C- 10C for up to one month.
4.2.31/500 nutrient broth
For 1 000 ml of purified water, take 3,0 g of meat extract, 10,0 g of peptone and 5,0 g of sodium chloride,put them in a flask and dissolve them thoroughly.Dilute this medium 500 times using distilled ordeionized water, and set the pH to (7,0 ± 0,2) using hydrochloric acid solution or sodium hydroxidesolution. Sterilize in an autoclave at 121C± 2°C for at least 15 min.After preparation, if 1/500 nutrientbroth is not used immediately, store it at 5 C to 10 c.
0ther culture media may be selected based on the requirements for the bacteria selected for the testing.Details of the selected media shall be included in the test report under culture conditions.
Prepared culture media may be stored at 5 C – 10°C for up to one month.
4.2.4 Nutrient agar
For 1 000 ml of purified water, take 3,0 g of meat extract, 5,0 g of peptone and 15,0 g of agar powder,put them in a flask and mix.Heat the flask in boiling water to dissolve the contents thoroughly. Use a0,1 mol/l solution of sodium hydroxide to bring the pH to (6,8 ± 0,2) at 25 °C. Sterilize in an autoclaveat 121C± 2 °C for at least 15 min. After preparation, if nutrient agar is not used immediately, store itat 5 °C to 10 °C.Keep the medium temperature between 45 °C and 48 °C when mixing with a bacterialsuspension.
Other culture media may be selected based on the requirements for the bacteria selected for the testing.Details of the selected media shall be included in the test report under culture conditions.
Prepared culture media may be stored at 5 C – 10 °C for up to one month.
4.2.5 Soybean-casein digest broth with lecithin and polysorbate 80 (SCDLP)
For 1 000 ml of purified water, take 17,0 g of casein peptone, 3,0 g of soybean peptone,5,0 g of sodiumchloride, 2,5 g of phosphoric acid monopotassium dehydrogenate, 2,5 g of glucose and 1,0 g of lecithin,put them in a flask and dissolve them.Add 7,0 g of non-ionic surfactant and dissolve it. Use a solution ofsodium hydroxide or hydrochloric acid to bring the pH to(7,0±0,2) at 25 °C.Sterilize in an autoclave at121C±2C for at least 15 min.After preparation, if sCDLP is not used immediately, store it at 5 °C to10 c.
Prepared SCDLP solution may be stored at 5 C – 10 °C for up to one month.
4.2.6 Phosphate buffer saline (PBS)
For 1 000 ml of purified water, take 34,0 g of potassium dihydrogen phosphate, put it in a flask anddissolve it thoroughly.Use a solution of sodium hydroxide to bring the pH to (7,0± 0,2) at 25 °C.Sterilizein an autoclave at 121C± 2 °C for at least 15 min. After preparation, if PBS is not used immediately,store it at 5 C to 10C.
Prepared PBS solution may be stored at 5 C – 10 °C for up to one month.
5 Test specimen
5.1 Size
Reduce samples to test pieces of a size approximately (50 ± 2) mm × (50 ± 2) mm square or as close to these dimensions as practical. Test specimen shall be sterilized based on the appropriate sterilization process selected for the test sample. All surfaces of the test specimen shall be sterilized.ISO 17721-1 pdf download.