BS EN 49-1-2016 pdf free download.Wood preservatives — Determination of the protective effectiveness against Anobium punctatum (De Geer) by egg-laying and larval survival Part 1 : Application by surface treatment (Laboratory method)
The treated test specimens are exposed to gravid females of Anobium punctatum. The number of eggs laid,the number of eggs hatched and the numbers of surviving larvae are observed and  ompared with those in untreated control test specimens. If the preservative has been prepared in the laboratory by dilution of a concentrate or by dissolution of a solid, the resulting attack is also compared to that in solvent or diluent treated control test specimens.
5 Test materials
5.1 Biological material
Anobium punctatum (De Geer)
Adult males and females in good condition.
Adults to be used in the test shall be collected at daily intervals from naturally infested wood or laboratory culture (see Annex C).
Use recently emerged adults which have been recently collected; kept overnight in quarantine (see C.6); and then checked to ensure that they are undamaged, active and free from any infestation by mites. Determine the sex (see Annex B) of the collected and checked adults and place the males and females in separate
containers.
NOTE The proportion of males and females varies during the emergence period.
5.2 Products and reagents
5.2.1 Paraffin wax, for sealing the relevant faces of test specimens to be treated with solutions in which water is the continuous phase.
NOTE Paraffin wax with a setting point of 52 °C to 54 °C has been found to be suitable.
5.2.2 Gelatin, for sealing the relevant faces of test specimens to be treated with solutions in which an organic solvent is the continuous phase.
5.2.3 Paste, for securing filter paper. The paste shall be starch-free, non-toxic to Anobium punctatum and insoluble in the product under test.
NOTE Sodium carboxy methyl cellulose, food grade, has been found to be suitable.
5.2.4 Water, complying with grade 3 of EN ISO 3696.
5.2.5 Solvent or diluent, a volatile liquid that will dissolve or dilute the preservative but does not leave a residue in the wood at the end of the post-treatment conditioning period that has a toxic effect on the insects.
CAUTION — Do not use benzene or other solvents which pose a health risk.
5.2.6 Filter paper, ordinary quality, medium-fast grade.
5.2.7 Fine cloth of a suitable material with a mesh aperture of 0,3 mm to 0,6 mm for the preparation of the egg-laying zones.
NOTE Cotton, linen and polyamide-gauze have been proven as suitable.
5.3 Apparatus
5.3.1 Culturing chamber, with air circulation, controlled at (21 ± 2) °C, and at relative humidity (80 ± 5) .
5.3.2 Conditioning chamber, well ventilated, controlled at (20 ± 2) °C and at relative humidity (65 ± 5) %.
The conditioning of test specimens may be carried out in the laboratory work area (see 5.3.4) provided that this has the conditions specified for the conditioning chamber (see 5.3.2).
5.3.3 Treatment vessel, of a material that does not react with the preservative under test, for example of glass for organic products and of polyethylene for salts containing fluorine.
5.3.4 Laboratory work area, well ventilated, where treatment of the test specimens is carried out.
CAUTION — It is essential to follow safety procedures for handling flammable and toxic materials.
Avoid excessive exposure of operators to solvents or their vapours.
5.3.5 Testing chamber, with conditions identical to those of the culturing chamber (see 5.3.1).
5.3.6 Pipette, of type specified in EN ISO 835, Class B: graduated pipette with no waiting time. Capacity 1 ml with an accuracy of ± 0,01 ml.
5.3.7 Safety equipment and protective clothing, appropriate for the test product and the test solvent, to ensure the safety of the operator.
5.3.8 Test containers, suitable for holding the test specimens and of material resistant to the solvents used, and fitted with perforated covers to provide a good exchange of air.
NOTE Jars of approximately 60 mm diameter and 100 mm height have been found to be suitable.
5.3.9 Ordinary laboratory equipment, including a balance capable of weighing to an accuracy of 0,01 g.
5.3.10 X-ray apparatus, (optional) with tungsten target and beryllium window, with voltage and current continuously variable in the ranges:
— voltage: 10 kV to 50 kV;
— current: 0 mA to 15 mA.
5.3.11 Protective gloves
6 Sampling
The sample of preservative shall be representative of the product to be tested. Samples shall be stored and handled in accordance with any written recommendations from the supplier.
For the sampling of preservatives from bulk supplies, the procedure given in EN 212 should be used.
7 Test specimens
7.1 Species of wood
The reference species is European oak. This shall be either sessile oak (Quercus petraea (Mattuschka) Lieblin) or pedunculate oak (Quercus robur Linnaeus).BS EN 49-1 pdf download.