BS EN 46-2-2016 pdf free download.Wood preservatives — Determination of the preventive action against recently hatched larvae of Hylotrupes bajulus (Linnaeus) Part 2: Ovicidal effect (laboratory method)
4 Principle
In this laboratory method treated wood panels are offered to freshly mated Hylotrupes bajulus females. The hatching ability of the larvae on the treated timber is examined. When the ovicidal action is insufficient, the mortality of the hatched larvae on and/or in wood treated with the same formulation is also established according to EN 46-1.
5 Test materials
5.1 Biological material
5.1.1 Hylotrupes bajulus (Linnaeus) females.
5.1.2 Source of females
The insects shall preferably be obtained from cultures reared as e.g. described in Annex B.
Use only sound and lively insects.
5.2 Products and reagents
5.2.1 Paraffin wax, for fixing the glass plate in all cases and for sealing the end faces of test specimens to be treated with solutions in all cases in which water is the continuous phase.
NOTE Paraffin wax with a setting point of 52 °C to 54 °C has been found to be suitable.
5.2.2 Gelatine, for sealing the end faces of test specimens to be treated with solutions in which an organic solvent is the continuous phase.
5.2.3 Water, complying with grade 3 of EN ISO 3696.
5.2.4 Solvent or diluent, a volatile liquid that will dissolve or dilute the preservative but does not leave a residue in the wood at the end of the post-treatment conditioning period that has a toxic effect on the insects.
CAUTION — Do not use benzene or other solvents which pose a health risk.
5.3 Apparatus
5.3.1 Culturing chamber, with air circulation, and controlled at (28 ± 2) °C and at relative humidity (70 ± 5) %.
5.3.2 Conditioning chamber, well ventilated and controlled at (20 ± 2) °C and at relative humidity (65 ± 5) %.
The conditioning of test specimens may be carried out in the laboratory work area (see 5.3.3) provided that this has the conditions specified for the conditioning chamber (see 5.3.2).
5.3.3 Laboratory work area, well ventilated, where treatment of the test specimens is carried out.
CAUTION — It is essential to follow safety procedures for handling flammable and toxic materials. Avoid excessive exposure of operators to solvents or their vapours.
5.3.4 Testing chamber, ventilated and air-conditioned, controlled at (22 ± 2) °C and at relative humidity between (70 ± 5) %.
5.3.5 Petri dishes of glass or polyvinylchloride (PVC), diameter ca. 9 cm for mating the insects and for egg-laying.
5.3.6 Weights, to provide ballast for the test specimens.
The weights shall not react with any materials with which they come into contact during the test.
5.3.7 Safety equipment, protective clothing, appropriate for the test product and the test solvent, to ensure the safety of the operator.
5.3.8 Glass plates, (48 ± 1) mm long and (25 ± 1) mm wide, intended to provide a lateral slit on the test specimens.
5.3.9 Ordinary laboratory equipment, including a balance capable of weighing to an accuracy of 0,01 g and equipment for applying a liquid product by brushing or by pipette.
5.3.10 Protective gloves
6 Sampling
The sample of preservative shall be representative of the product to be tested. Samples shall be stored and handled in accordance with any written recommendations from the supplier.
For the sampling of preservatives from bulk supplies, the procedure given in EN 212 should be used.
7 Test specimens
7.1 Species of wood
The reference species is Scots pine (Pinus sylvestris Linnaeus) 1) .
Additional tests may be carried out using other species but, if so, this shall be stated in the test report.
7.2 Wood quality
The wood shall be free from visible cracks, stain, decay, insect damage and other defects. The wood shall not have been water-stored, floated, chemically treated or steamed. The wood shall originate from trees preferably felled in winter. The trees shall be cut immediately after felling and the timber rapidly air dried or kiln dried at temperatures below 60 °C. The wood shall not have been stored for more than five years. The wood shall be exclusively sapwood containing little resin and having between 2,5 annual rings per 10 mm and eight annual rings per 10 mm. The proportion of latewood in the annual rings shall not exceed 30 % of the whole.+
It is recommended to use test specimens of similar growth rate within a single test.
7.3 Provision of test specimens 2)
Prepare planed strips having a cross-section of (25 ± 0,5) mm × (15 ± 0,5) mm removing a minimum of 2 mm from any surfaces exposed during drying. The longitudinal faces shall be parallel to the direction of the grain. The annual rings shall have a contact angle of 45° ± 15° to the broad faces. Make transverse cuts, neatly to give sharp edges and a fine-sawn finish to the end-grain surfaces, to give test specimens (50 ± 0,5) mm long.
The test specimens shall originate from a minimum of three trees or shall be taken at random from a stock originally of more than 500 test specimens.BS EN 46-2 pdf download.